Genome Editing Scientist

Genome Editing Scientist

Qualifications

• Molecular Biology or Cell Biology Major Highly preferred
• Three (3) years of relevant post-degree work experience or in biology, cell-biology, molecular biology, genetics, bioengineering, or a related discipline is required with a Master’s degree

• Two (2) years of relevant post-degree work experience in biology, cell-biology, molecular biology, genetics, bioengineering, or a related discipline is required with a Ph.D

• Expertise and hands-on experience with primary mammalian cell culture and cell biology

• The ideal candidate will have experience with genome editing technologies, espcially Prime Editing; knowledge of gene editing techniques including CRISPR-Cas9, Prime Editing, AAV biology, and/or genome editor delivery.

• Hands-on experience or working knowledge of qPCR techniques

• Experience in Human Induced Pluripotent Stem Cells(hiPSC), or organoids preferred

• Comfortable working in a dynamic research and early-discovery environment, as well as collaborating with peers on multiple projects at once

• Excellent oral and written communication skills, and ability to simplify complex biological concepts to peers

• Exceptional communication and presentation skills

• Excellent trouble shooting, capability, attention to detail and ability to work independently as well as part of a team

Responsibilities

• Optimizing pre-clinical gene editing strategies for treating genetic muscular disease, which is Limb-Girdle Muscular Dystrophy(LGMD) Type 2B

• Developing strong pre-clinical data to support advancing potentially curative, autologous cell therapy approaches to treating LGMD2B

• Protein engineering of genome editors with improved activity, targeting range, or specificities

• The bench Scientist will also be responsible for contributing intellectually and hands-on to other research, early-discovery activities, and core capabilities at ACGENE Lab

• Culture and edit hiPSCs and characterize the levels of editing by flow cytometry, and qPCR

• Develop multicolor or imaging-based flow cytometry assays, western blot, and/or molecular assays to assess the effects of editing on cell biology and fitness

• Design and optimize Prime Editor (Gene Editor)
• Plasmids Prep. and Verification

• Produce, purify, and characterize AAV vectors

• Analyze data and present results in cross-functional team meetings

• Establish and contribute positivity to the broader vision of company culture

• Design and evaluate gene editing constructs in stem cells

• Establish and perform molecular assays to determine on target gene editing efficacy

Job Type: Full-time

Pay: $40.00 - $45.00 per hour

Education:

• Master's (Required)

Experience:

• Molecular biology: 3 years (Required)
• Genome Editing: 3 years (Required)

Work Location: One location