What are the responsibilities and job description for the Scientist- Phage Display position at Actalent?
Description:
• Use expertise in molecular biology to generate Chimeric Antigen Receptor (CAR) or antibody constructs.
• Engineer lentiviral vectors with insertion or deletion of restriction sites, epitope tags or other genetic elements.
• Generate viral vectors expressing scFv or CAR variants, site directed mutants, and fusion proteins using fusion PCR and Gibson or other fragment assembly methods.
• Design and clone shRNA, gRNA for desired targets in CRISPR/Cas9 vectors.
• Design strategies to knock-in CAR in T cells
• (4) Perform antibody phage display screens and validation of clones by ELISA and flow cytometry.
• Express/Purify antibodies and recombinant proteins in bacteria or mammalian cells.
• Develop biochemical, cell based and immunoassays to evaluate antibody binding affinities
• Optimize qPCR/ddPCR assays to characterize viral and CAR-T products.
• Construct phage display libraries
• Perform restriction analysis, ligation, transformation, colony PCR screens and gel electrophoresis.
• Develop generate stable cell lines with overexpression or knock out (CRISPR shRNA).
• Prepare plasmids in small scale and large scale, maintain plasmid and bacterial glycerol stocks.
• Maintain cell lines, prepare cell banks (adherent and suspension cell lines) as required.
• Perform cytokine ELISAs and Western blotting.
• Perform cell-based (proliferation, cytotoxicity, transcription factor reporter) assays in multi well formats, analyze and clearly document data.
Knowledge, Skills & Abilities:
• Communicate ideas and results clearly and effectively (both verbally and in writing) to the team members.
• Must feel comfortable in a fast-paced small company environment with minimal direction and able to adjust workload based upon changing priorities
• Ability to adapt to startup environment and make the best use of the existing resources to helping the team to move towards perfection
• Familiarity with routine lab operations and safety procedures.
• Self-motivated, pays attention to details, and willing to accept responsibilities outside of the initial job description
• Perform experiments with minimal supervision, think critically and demonstrate troubleshooting and problem-solving skills.
• Develop precise SOPs complying with GLP environment and maintain excellent records that allow systematic tracking and protocol transfer.
• Basic proficiency in various software (Excel, PowerPoint, Graph Pad Prism, Photoshop and Plasmid design/analysis software)
• Professional Knowledge
• Problem Solving
• Team Player / Building Relationships
• Multi‐tasking
• Customer Focus
• Action and detail oriented
• Active Listening
• Decisive Decision Making
Education & Professional Experience:
• MS Degree preferably with at least 5 years of laboratory experience or PhD with 2 years of laboratory experience in the field of Immunology, cell biology, virology, or molecular biology.
• Experience in phage display library construction, antibody engineering, humanization or affinity maturation of antibodies are preferred.
• Strong technical skills in advanced molecular cloning and fragment assembly techniques are required
• Strong experience in cell based and biochemical assays are required – must be able to perform assays in 96 and 384 well formats with different formats without confusion.
• Experience in working with multiple cell lines (both suspension and adherent) and setting up cell-based assays.
• General lab experience in operating and maintaining basic lab equipment (centrifuge, microscope, PCR machines, nanodrop, DNA and protein electrophoresis systems).
• Hands on experience with sterile microbiology and molecular biology techniques not limited to cloning, mutagenesis, restriction digestion, ligation, transformation, colony PCR, culturing bacterial cells, plasmid preparations.
• Critical to have the ability to maintain accurate records and back track samples prepared without confusion.
• Prior experience in optimizing and trouble-shooting qPCR and ddPCR
About Actalent:
Actalent connects passion with purpose. Our scalable talent solutions and services capabilities drive value and results and provide the expertise to help our customers achieve more. Every day, our experts around the globe are making an impact. We’re supporting critical initiatives in engineering and sciences that advance how companies serve the world. Actalent promotes consultant care and engagement through experiences that enable continuous development. Our people are the difference. Actalent is an operating company of Allegis Group, the global leader in talent solutions.
The company is an equal opportunity employer and will consider all applications without regards to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.
