Research Technician

Department: EMB – Embryology
Salary: TBD
Location: Baltimore, MD

Job Summary:
The Kostova laboratory at Carnegie’s Department of Embryology in Baltimore is searching for a full-time research technician. This is a great opportunity to work with a postdoctoral fellow using cutting-edge technologies to answer fundamental questions of cell biology. Ideal candidates should have a B.S. in Biology or a related field, experience in modern molecular biology techniques, a strong work ethic, and be able to join the position for at least 2 years.
We are investigating the fundamental question of how different types of ribosomes regulate translation and embryogenesis. Despite ribosomes’ importance, they are traditionally seen with minimal regulatory activity. Exciting new studies challenge this concept and provide evidence that organisms assemble ribosomes with different makeups during development in response to stress, including the development of multiple cancers. Although ribosome heterogeneity is hypothesized to alter translation, little experimental data exist to demonstrate this. The zebrafish embryo provides a power system to tackle this question. We use cutting-edge technology, such as genome editing and high throughput sequencing technology, to tag different ribosome populations and determine their ability to synthesize proteins.
We are looking for a highly motivated individual with strong organizational skills to join our team and help explore these novel areas of biology.
Key Responsibilities:

• Perform genome editing and characterize zebrafish strains (genotyping, qPCR, Western blotting)
• Isolation of ribosomes and RNA analysis (sucrose gradient fractionation, immunoprecipitation, RNA purification, and electrophoresis)
• Maintenance and upkeep of zebrafish-related infrastructure

Requirements:

• B.S. in Biology (or equivalent)
• Lab experience
• Strong interpersonal skills and ability to work as part of a team

Preferred Skills:

• Modern molecular biology techniques, e.g., DNA/RNA purification, cDNA synthesis, qPCR, plasmid cloning, SDSPAGE, and Western blotting
• Some experience working with zebrafish or other vertebrates in a research laboratory.
• Great organizational skills
• Excellent written and oral communication skills

If interested please send the following documents to kostova@carnegiescience.edu:

• Cover letter
• Curriculum Vitae
• Academic transcript
• Recommendation letters (at least 2)

Research Environment:
The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.
Additional Information:
Carnegie is an equal opportunity employer. All applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status, or any other characteristics protected by law.
At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

Job Type: Full-time