Visiting Graduate Student

School of Medicine

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Visiting Graduate Student POSITION IN PRE-CLINIC HYPERTENSION

RESEARCH– DUKE UNIVERSITY

Dr. Yang’s lab at Duke Department of Medicine has an NIH/NIDDK-funded position for

a postdoctoral fellow to study the mechanisms underlying hypertension pathogenesis and

hypertension-associated end-organ damage.

Responsibilities

• Performs various complex technical duties involved in conducting chemical, biological
  
  

and other research laboratory tests, experiments and determinations to obtain data for

research purposes; compile, analyze, document and draw tentative conclusions from

experimental results.

• Managing transgenic mouse colonies
• Confer with the principal investigator to review work assignments and develop plans for
  
  

research experiments; make minor modifications to existing laboratory procedures and

techniques to meet the needs of the particular equipment as required.

• Review laboratory methods manual, scientific journals, abstracts, and other literature
  
  

for information applicable to research experiments.

• Determine and set up instruments, materials, and apparatus and operate laboratory
  
  

equipment required for specific tests.

• Obtain, prepare, mount, and stain tissue, blood, and other substances for microscopic
  
  

examination.

• Prepare culture media, chemicals, reagents, and solutions.
• Maintain sufficient inventory of material, supplies, and equipment for performing
  
  

duties; clean and maintain laboratory equipment.

• Review the work of lower-level laboratory personnel within designated areas and assist
  
  

in training new personnel.

• Perform other related duties incidental to the work described herein.
• Review the completeness or accuracy of the work of others
• Give recommendations, advice, or guidance to others
• Review expenses related to materials and supplies and confirm the grant-budget
  
  

monthly reports.

• Generating reports of results
• Create and maintain laboratory records (protocols and biological materials)
  
  

Skills

• Knowledge/experience in various methods of molecular biology, analysis of gene
  
  

expression, genetics, functional genomics, cell biology and bioinformatics. For

example: samples receipt and handling, RNA-seq, real-time PCR, RNA handling, RTPCR,

qPCR, cell-culture, viral transduction, transfections, luciferase reporter assays, GFP

reporter assay and immunofluorescence, RNA and DNA extraction, protein extraction

and analysis, nuclear extraction, chromatin immuno-precipitation, electrophoretic

mobility shift assay, DNA-binding protein purification, sequence analysis, genotyping,

bioinformatic tools for genomic analysis.

Minimum Qualifications

Education

Experience

See job description for education requirements.

See Job Description For Requirements.

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