Scientist III

Job C

Job Summary
Our purpose is to help all people live healthy lives. We are a world leader bringing innovative tools to research and clinical laboratories in basic research, drug discovery and development, biopharmaceutical production and disease management. We develop technology that enables faster diagnosis and better clinical outcomes.
To ensure that our cell analysis products deliver outstanding performance and exceed our customers’ expectations, BD Biosciences is seeking a passionate and capable Systems Validation Scientist II to contribute to the R&D team by preparing samples for flow cytometric studies.

Must haves on the resume:
1. Best practice experience doing antibody methods for antigen labeling (e.g., flow cytometry, western blots, ELISA, ELISPOT)
2. Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
3. Ability to purify leukocytes from mammalian-derived samples (i.e., venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.
4. Ability to stain cells in tubes and plates for intracellular and extracellular antigens.

Responsibilities:
The Systems Validation Scientist III will play a critical role in preparing samples for flow cytometric studies.

Responsible for:

• Maintaining the health, expansion, and inventory of cell lines
• Performing cryopreservation and thawing of mammalian cells
• Purification of leukocytes from mammalian-derived samples (i.e., venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.
• Staining of cells in tubes and plates for intracellular and extracellular antigens.
• Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents.
• Counting cells via hemocytometer and/or proprietary cell counters.
• Preparing buffers, media, and other reagents.
• Maintaining inventory of critical laboratory supplies (consumables and reagents) in the lab
• Attend meetings, as needed.

Qualifications:
The successful candidate will possess:

• A minimum of a bachelor’s degree in Microbiology, Biology, Biochemistry, Immunology, or a related field.
• At least 2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines.
• Best practice experience doing antibody methods for antigen labeling (e.g., flow cytometry, western blots, ELISA, ELISPOT)
• Experience working with primary cells derived from various mammalian species.
• Proficient with phase-contrast and brightfield microscopy.
• Excellent aseptic technique.
• Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies.
• Abibity to calculate basic laboratory calculations.
• Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency.
• Ability to read and understand Standard Operating Procedures
• Ability to interact with researchers for delivery of cells.
• Ability to maintain a clean work area.
• Knowledge of GLP/GDP guidelines.
• Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
• Excellent written and oral communication skills.

Job Type: Contract

Salary: $40.00 - $50.00 per hour

Schedule:

• 8 hour shift
• Monday to Friday

Ability to commute/relocate:

• San Jose, CA 95131: Reliably commute or planning to relocate before starting work (Preferred)

Experience:

• Biosafety Level 2: 2 years (Required)
• Mammalian cell culture: 2 years (Required)
• Flow cytometry: 2 years (Required)

Work Location: One location