What are the responsibilities and job description for the Regenerative Medicine Post Doc position at CRISPR Therapeutics?
Company Overview
CRISPR Therapeutics is a leading gene editing company focused on developing transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform. CRISPR/Cas9 is a revolutionary gene editing technology that allows for precise, directed changes to genomic DNA. CRISPR Therapeutics has established a portfolio of therapeutic programs across a broad range of disease areas including hemoglobinopathies, oncology, regenerative medicine, and rare diseases. To accelerate and expand its efforts, CRISPR Therapeutics has established strategic collaborations with leading companies including Bayer, Vertex Pharmaceuticals and ViaCyte, Inc. CRISPR Therapeutics AG is headquartered in Zug, Switzerland, with its wholly-owned U.S. subsidiary, CRISPR Therapeutics, Inc., and R&D operations based in Cambridge, Massachusetts, and business offices in San Francisco, California and London, United Kingdom.
Job Summary
Differentiation of induced pluripotent stem cells (iPSCs) to T-cells is a new area for regenerative medicine that we would like to explore. The combination of iPSC and CAR-T technologies creates the ability to potentially manufacture iPSC-derived CAR-T (CAR-iT) therapeutics that are potentially much cheaper, more reproducible, and more efficacious at killing cancer than primary cell derived CAR-Ts. The postdoc will be expected to evaluate and reproduce published iPSC to T-cell differentiation protocols as well as make their own optimizations to the protocols based on literature and experimental data. Once an iPSC to T-cell differentiation protocol is established, stem cell derived CAR-iT cells would be generated, characterized, and compared against primary cell derived CAR-Ts to assess the benefits and/or challenges of CAR-iTs. Please note, this job is paid in accordance with industry standards.
Responsibilities
- Optimize/develop iPSC to T-cell differentiation protocols
- Characterize iPSC-derived T-cells (iT cells) in terms of gene expression, killing ability, manufacturing capability
- Generate CAR-iT cells and compare them against primary cell derived CAR-T cells in killing assays in vitro and in vivo
Minimum Qualifications
- PhD in molecular or cell biology or equivalent field
- Excellent oral and written communication skills
- Ability to work independently as well as collaboratively in a results-oriented research team environment
- Self-motivated with critical thinking skills
- You must be legally authorized to work in the United States during the entire length of the postdoc
Preferred Qualifications
- Experience with iPSC differentiation
- Significant experience with various molecular cloning techniques such as restriction enzyme and Gibson Assembly cloning
- Experience with gene editing of mammalian cell lines
- Experience with T-cells/NK cells
CRISPR Therapeutics, Inc. is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation or any characteristic protected under applicable law.
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