Associate Scientist

Position Details:

Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Associate Scientist to join their expanding team.

Job Title: Associate Scientist / Mammalian Cell Culture / Pharma Industry

Duration: 18 months contract, extendable up to 36 months

Location: Cambridge, MA

Note:

The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.

You may participate in the company group medical insurance plan

Job Description:

Associate Scientist

Summary:

The Target Protein Science group within BioMedicine Design Department based in Cambridge, MA is seeking a meticulous and organized individual with previous experience in cell culture to assist with daily maintenance of 10 cell lines to support discovery and testing of therapeutic antibodies across various disease areas.

This individual will be responsible for laboratory instrument maintenance and oversee supplies within the cell biology laboratory, in addition to assisting in development and characterization of target protein overexpression lines, as well as CRISPR KO/KI lines.

Working with experienced cell and molecular biologists, this position offers an opportunity to learn methods for stable cell line generation, including cell based gene editing, flow cytometry-based sorting and analysis, and more traditional cell characterization techniques such as Western blot and immunocytochemical analysis.

Responsibilities:

Creation and characterization of stable mammalian cell lines for the production of target proteins for antibody discovery and validation

Culturing and maintenance of a variety of mammalian cell lines (adherent and suspension cultures), and insect cells

Oversee supplies, instrument calibration and maintenance

Perform transfections, selections, screening, cloning, and characterization of stable cell lines

Characterization and documentation of expression results using flow cytometry analysis, gel electrophoresis, immunoblotting, and other relevant techniques

Coordinate cell line deliveries with various project team members

Coordinate receiving and delivering, mycoplasma testing, and cryopreservation of cell lines

Cryofreezer organization and maintenance

Qualifications:

BS/BA with a minimum of 0-3 years of experience in a laboratory setting

Experience with cell cultures / sterile technique

Experience with protein and Western Blot

Experience with Mammalian cell lines

Experience with flow cytometry and assays preferred

Data analysis experience and proficiency

Willingness to learn and adopt new techniques

Organized individual with strong record-keeping capabilities

Attention to detail and ability to multitask

Location:

This is a fully onsite role located in Cambridge, MA