RESEARCH TECHNICIAN - Mravic Lab

ABOUT US:

Scripps Research is ranked one of the most influential institutions in the world for its impact on innovation. We expand basic knowledge in the biosciences, and use these fundamental advancements to develop profound innovations that improve wellbeing. Our educational and training programs mold talented and committed students and postdocs into leading edge scientists.  Scientists in the institute’s six academic research departments work hand-in-hand with researchers of the Scripps Research Translational Institute and Calibr to merge foundational studies in biology, chemistry and computer science with translational science to produce pioneering drugs and advances in digital and precision medicine. Together, we cultivate new scientific leaders and expand the frontiers of knowledge to deliver medical breakthroughs to better human health around the globe. If you have a passion for making a difference, this could be your opportunity to join our transformative team.

POSITION TITLE:  Research Technician

POSITION SUMMARY:

Perform a variety of routine scientific tasks; may prepare test specimens; set up and operate standard lab equipment of moderate complexity.

The Mravic Lab is a molecular biophysics and protein engineering laboratory seeking to hire a highly motivated full-time Research Technician to perform cutting-edge molecular characterization of synthetic transmembrane proteins designed by computational tools for biotechnology applications. 

The scientist will train and execute a suite of experimental techniques to produce then study the biochemical structure and function of computationally designed proteins.  This will include enacting lab standard protocols, fast-paced adaptation to optimize or troubleshoot techniques to new challenges, and developing new laboratory procedures under guidance of senior scientists.  The research is multi-disciplinary in nature, thus the ability to adapt and a willingness to learn new methods are essential.  Additional opportunities for training in computational biology and characterization of synthetic proteins within living cells may be available for those candidates with previous skills in coding and mammalian cell culture, respectively. 

RESPONSIBILITIES AND DUTIES:

General

• Perform research and conduct a wide variety of standard non-repetitive laboratory procedures under minimal supervision, independently and in collaboration with others.
• Record and analyze data, and interpret results
• Investigate, troubleshoot and modify methods and procedures as necessary; may assist in designing experiments
• Perform laboratory support tasks for the group and project: routine equipment maintenance, preparing common reagents, organization, ordering materials, etc.
• Details of essential functions for this position will be discussed during the interview process
• Impeccable record keeping via laboratory notebook documentation and data management
• Contribute to academic research publications; preparing manuscript figures, writing methodology sections, and supplemental material

Technical

• Molecular biology, DNA cloning, transformation of laboratory e. coli strains
• Recombinant expression and purification of synthetic transmembrane proteins in aqueous detergent conditions: His-tag, Ion exchange, Size-exclusion FPLC
• Reverse phase high performance liquid chromatography (RP-HPLC) of proteins / peptide in organic solvent
• Analytical characterization of proteins / peptides: SDS-PAGE, liquid chromatography mass spectrometry (LCMS), western blot, fluorescence spectroscopy
• Biophysical / biochemical assays e.g. characterization of protein-protein interactions
• Protein crystallography with assistance of high-throughput robotics; X-ray synchrotron experiments at national laboratory particle accelerators
• Preparation of samples for structural biology: Cryo-electron microscopy, X-ray crystallography, NMR

REQUIREMENTS:

• Requires a bachelor's degree in a relevant scientific discipline and up to one (1) year of related experience.  
• Three (3) to six (6) years of related laboratory experience may be considered in lieu of a degree. 

 PREFERRED QUALIFICATIONS: 

• Bachelor’s degree in biochemistry, bioengineering, chemistry, molecular biology, or related field.
• Undergraduate level understanding of molecular biology and protein chemistry
• Previous experience with DNA cloning and expression, purification, and analytical characterization of protein / peptide molecules in a research laboratory setting
• Strong preference for candidates with background with protein chemistry, membrane proteins, and/or protein crystallography
• Strong oral and written communication, data documentation, and presentation skills
• Ability to multi-task across different research directions day-to-day
• Excellent collaborative and interpersonal skills

 COMPENSATION:

The expected hiring range for this position is $18.67 to $24 per hour, commensurate with experience. Consideration will be given to experience that exceeds the listed requirements.

COMPREHENSIVE BENEFITS INCLUDE: 

• Employer Contributed Retirement Plan – Depending on eligibility, employees receive an employer contributed retirement plan (no employee contribution required) and the option to contribute to a 403(b) (which is similar to a 401(k) using your own pre or post-tax dollars)
• Full Suite of Health and Welfare plans including three medical plan options (including an HSA available option), dental, vision, life insurance, disability, EAP and more
• Access to Flexible Spending Accounts (Medical/Dependent Care)
• Competitive vacation and sick leave policies
• Free, on-site parking

The above statements describe the level of work performed and expected in general terms.  The statements are not intended to list all the responsibilities, duties and/or skills required of employees so classified, and the content herein is subject to change due to the business needs of Scripps Research, with or without notice.   Furthermore, nothing in this job description shall be interpreted to be in conflict with or to eliminate or modify in any way the employment-at-will status of Scripps Research staff. 

EEO Statement:
The Scripps Research Institute is an Equal Opportunity Employer. We promote diversity of thought, culture and background in the fields of science. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other legally protected characteristic or status. Underrepresented individuals are encouraged to apply.